PMID: 10329377

Detection of DNA abnormalities by arbitrarily primed PCR fingerprinting : amplification of the MDM2 gene in a mediastinum fibrosarcoma .

Arbitrarily primed PCR ( AP-PCR ) fingerprinting method is easy and useful for analysis of genetic alterations in anonymous chromosomal regions .

We applied this technology to analysis of DNA from human primary cancers and found amplification of a DNA fragment in a mediastinum fibrosarcoma .

PCR-based analysis of radiation hybrid panels following cloning and nucleotide sequence determination of the fragment revealed that it was derived from a region of chromosome 12q13-q15 .

In this region , the MDM2 and IFNG genes were noted as known genes that could be involved in human carcinogenesis .

Southern blot analysis of genomic DNA of the tumor revealed the amplification of the MDM2 gene together with the fragment locus , but not the IFNG gene .

Our results demonstrated that detection of DNA alterations by AP-PCR fingerprinting without any previous knowledge of the genes and subsequent analysis of radiation hybrid panels could lead to easy identification of candidates for genes involved in carcinogenesis .